Figure 4.

Effects of MANF on Cell Migration from the SVZ Explants

(A) Representative images of SVZ explants at DIV2 and DIV7 from WT and Manf^−/− embryos. (B) MANF-deficient cells from the SVZ explants migrate shorter distances than WT cells at DIV7 (two-way ANOVA, genotype X time effect p = 0.02; **p < 0.01 TK, n = 7 to 8). (C) 64 SVZ explants treated with rhMANF (0, 200, 400, and 800 ng/mL) or rhBDNF (25 ng/mL) from day 1 to day 4 and representative photomicrographs are shown from DIV1 and DIV7. (D) BDNF and MANF treatments significantly increased the migration of cells (n = 7 to 8, two-way ANOVA, treatment × time p < 0.01). At DIV4, the migration of cells was significantly increased from BDNF and rhMANF (400 ng/mL) treated SVZ explant (**p < 0.05, TK). At DIV7, BDNF and rhMANF (200 and 400 ng/mL) treatments enhanced the distance of migration (***p < 0.001 TK). Scale bars, 500 μm (A) and 1,000 μm (C). TK, Tukey’s post hoc test. Data are presented as mean ± SEM.