Figure 8.

MANF Injections, 3 × 10 μg, Resulted in Increased Numbers of Neuroblasts in the Corpus Callosum after Stroke

(A) Distribution of DCX⁺/BrdU⁺ cells in the corpus callosum beneath the infarction zone. (B) Number of DCX⁺/BrdU⁺ cells in the ipsilateral corpus callosum increased due to MANF treatment (n = 5 in all groups, one-way ANOVA p < 0.001, **p < 0.01, ***p < 0.001 TK). (C) GFAP immunostaining showed that most BrdU-labeled cells also express GFAP in the infarction zone. (D) The number of GFAP⁺/BrdU⁺ cells in the infarction zone did not differ between groups. (E) NG2⁺ cells expressing BrdU within the infarct boundary. (F) Number of BrdU⁺/NG2⁺ cells in the corpus callosum beneath the infarction zone did not differ between treatment groups; scale bars, 50 μm. Data are presented as mean ± SEM.