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. 2017 Sep 21;26(1):238–255. doi: 10.1016/j.ymthe.2017.09.019

Figure 9.

Figure 9

Effect of Long-Term GDNF and MANF, 0.25 μg/μL, a total of 200 μL, Infusion in the Peri-infarction Zone after Cortical Stroke

(A) A timeline of the experiment. (B) Representative photomicrographs of infarction areas in different groups. (C) Staining and quantification of lesioned brain areas show similar infarction sizes between groups, n = 5. (D–H) DCX+ cells after PBS, GDNF, or MANF administration in the SVZ (D and E), corpus callosum (F and G), and infraction zone (H). (I) GDNF and MANF treatments increase the number of DCX+ cells in the infarction zone (n = 4 to 5, one-way ANOVA p < 0.01, **p < 0.01 TK). (J) Images of NeuN (green) and BrdU (red) double-labeled cells in the peri-infarction zone. (K) A trend toward increase in the number of new mature cortical neurons in the peri-infarction zone with MANF treatment. n = 5. Scale bars, 2,000 μL (B), 50 μm (D–H), and 20 μm (J). Data are presented as mean ± SEM.