Figure 4.

Representative electron microscopy (EM) images of mitochondrial morphology in the brain cortex after ROSC. (a) Electron micrograph of mitochondria in the brain cortex at 4 h, 12 h, and 24 h after ROSC. Scale bar = 1 μm. (b) Mitochondrial aspect ratio was measured by the line tool and then the ranges were calculated and converted to their actual values using the scale bar. (c) Mitochondrial number was counted by two blinded observers and normalized to unit field. n > 300 mitochondria in each group. Data are represented as mean ± SD. ^∗P < 0.05, ^∗∗P < 0.01 versus sham.