Figure 2.

Aptamer-Dependent Inhibition of CCL17-Dependent Cell Migration In Vitro

Migration of BW5147.3 cells toward 100 ng/mL (7.5 pmol/well) mCCL17 was measured in a transwell system. The full-size and unmodified aptamers MF11 and MF35, as well as the RNA pool from selection cycle 1, were tested at equimolar concentrations (7.5 pmol) and at an aptamer/CCL17 ratio of 1:10 (0.75 pmol). As a control, 7.5 pmol of an anti-CCL17 monoclonal antibody (1.88 μg/mL) was added to the lower compartment. After 2 hr, transmigrated cells in the lower compartment were counted and calculated as percent of CCL17 alone (n = 3–5, mean ± SEM). Statistical significance was tested by one-way ANOVA with Bonferroni post hoc test for multiple comparisons (***p < 0.001).