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. 2017 Oct 13;26(1):95–104. doi: 10.1016/j.ymthe.2017.10.005

Figure 4.

Figure 4

MF11.46.m and MF35.47.m Efficiently Inhibit CCL17-Dependent Cell Migration In Vitro

The ability of the shortened and modified (5′-PEGylated, 3′-capped) aptamers to inhibit CCL17-dependent migration of BW5147.3 was measured in a transwell system. MF11.46.m and MF35.47.m were tested at a molar ratio of 1:1 (7.5 pmol), 1:10 (0.75 pmol), and 1:100 (0.075 pmol) (aptamer:mCCL17). An equally modified scrambled control aptamer served as a negative control. After 2 hr, cells in the lower compartment were counted and calculated as percent of migration toward CCL17 alone (n = 3, mean ± SEM). Statistical significance was tested by one-way ANOVA with Bonferroni post hoc test for multiple comparisons (**p < 0.01; *p < 0.05).