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. 2017 Oct 10;26(1):84–94. doi: 10.1016/j.ymthe.2017.10.003

Figure 2.

Figure 2

Systemic Administration of scL-Based Nanocomplex Carrying the GFP Gene Results in Transgene Expression in Mouse Brain

(A) Mice were intravenously injected once with controls or scL carrying GFP plasmid DNA (30 μg of GFP plasmid DNA/mouse). Maestro imaging of mouse brain was performed at 48 hr after the single tail-vein injection. The fluorescence image is overlaid on the bright-field image in mid-sagittal view. (B–D) Other mice were repeatedly intravenously injected with scL-GFP (at 30 μg of GFP plasmid DNA/injection/mouse), i.e., every 12 hr for a total of three injections. (B) Maestro imaging of serial coronal sections of mouse brain was performed at 24, 36, and 48 hr after the last injection. (C) GFP fluorescence quantitated and plotted as a function of time. Data are represented as mean ± SD (n = 2 mice per each time point). (D) Higher-resolution immunofluorescence microscopy confirmed GFP expression in hippocampus (CA1) and cortex of mouse brain at 48 hr after the last injection of scL-GFP. Red arrows, blood vessels; white arrows, neurons; yellow arrows, possible neurons, glia, or cross-section of vessels. Scale bar, 75 μm.