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. 2017 Oct 10;26(1):84–94. doi: 10.1016/j.ymthe.2017.10.003

Figure 5.

Figure 5

scL Suppresses TNF-α Production in Cell Culture and Delivers siRNA into Mouse Brain Cells In Vivo

(A and B) Raw 264.7 cells were transfected with siTNF or control siRNA complexed with scL followed by LPS treatment 24 hr after transfection. TNF-α mRNA levels (A) in cells and secreted TNF-α protein (B) in culture medium were assessed in triplicate 4 hr after LPS treatment. Data are represented as mean ± SD. *p < 0.001, **p < 0.05. (C) Quantitative analysis of the siTNF uptake in mouse brain cells using RT-PCR. Mice were intravenously injected with scL-siTNF nanocomplexes (180 μg of siRNA/mouse). At 24 hr after a single injection, the physical presence of siTNF was determined. Data are represented as mean ± SD (n = 2 to 3 mice per group). *p < 0.001.