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. 2017 Oct 10;26(1):84–94. doi: 10.1016/j.ymthe.2017.10.003

Figure 6.

Figure 6

scL-Delivered TNF-α siRNA Protects Mice in Lethal Endotoxemia In Vivo

(A) Mice were intravenously injected with scL-siTNF nanocomplexes and challenged with LPS as indicated. PBS was injected as a control. (B and C) At 1 hr after LPS injection, the changes in the level of TNF-α mRNA (B) and TNF-α protein (C) in mouse brains were assessed by RT-PCR and ELISA. Data are represented as mean ± SD (n = 5 to 10 mice per group). *p < 0.001, **p < 0.05. (D) Survival was assessed at 24 hr after intraperitoneal injection of LPS (n = 10 mice per group). (E) Representative images of TUNEL staining of brain 24 hr after LPS injection (20× magnification). Scale bar, 200 μm.