Table 1.

Lentiviral and Retroviral Vector Lots, RCL/R Monitoring

Investigational Product	Vector Type	Envelope	Promoter	Transgene	Number of Vector Lots				NCT Number(s)
					Produced	Tested for RCR/L via Biological Method	Tested for RCR/L via Molecular Method	Used for T Cell Product Manufacture
CTL019	lentivirus, third generation, SIN	VSV-G	EF-1a	murine anti-CD19 scFv with 4-1BB and CD3ζ signaling domains	9	9	8	7	NCT02030847, NCT01029366, NCT01626495, NCT01747486, NCT02135406, NCT02030834, NCT02465983
CTL119	lentivirus, third generation, SIN	VSV-G	EF-1a	humanized anti-CD19 scFv with 4-1BB and CD3ζ signaling domains	2	2	1	3	NCT02374333, NCT02640209, NCT02030834
CART-meso	lentivirus, third generation, SIN	VSV-G	EF-1a	murine anti-mesothelin scFv with 4-1BB and CD3ζ signaling domains	1	1	1	2	NCT02465983, NCT02159716
CART-BCMA	lentivirus, third generation, SIN	VSV-G	EF-1a	human anti-BCMA scFV with 4-1BB and CD3ζ signaling domains	1	1	0	1	NCT02546167
CART-EGFRvIII	lentivirus, third generation, SIN	VSV-G	EF-1a	humanized anti-EGFRvIII scFV with 4-1BB and CD3ζ signaling domains	1	1	1	1	NCT02209376
VRX-496	lentivirus, third generation, SIN	VSV-G	HIV-1 LTR	antisense sequence targeting HIV env	1	1	0	1	NCT00295477
CD4zeta	retrovirus, second generation	aMLV	PGK	human CD4 transmembrane and extracellular domains fused with CD3ζ signaling domain	1	1	0	1	NCT01013415
MazF	retrovirus, second generation	GaLV	HIV-1 LTR	MazF endoribonuclease	1	1	0	1	NCT01787994
Lentivirus totals					15	15	11	15
Retrovirus totals					2	2	0	2

SIN, self-inactivating; VSV-G, vesicular stomatitis virus glycoprotein; LTR, long terminal repeat; PGK, phosphoglycerol kinase; aMLV, amphotropic murine leukemia virus; GaLV, gibbon ape leukemia virus.