Figure 3.

p70^S6K siRNA/G₆ Dendriplex-Mediated Gene Silencing and Inhibition of Cell Viability of Ovarian SKOV-3 CSCs, HEYA8 CSCs, and SKOV-3 Non-CSCs without Adverse Toxicity to Normal Human OSE Cells

(A, B, D, and F) p70^S6K protein levels of (A and B) SKOV-3 CSCs, (D) SKOV-3 non-CSCs, and (F) HEYA8 CSCs were measured after transfection with indicated solutions for 72 hr. (C, E, and G) Viability of (C) SKOV-3 CSCs, (E) SKOV-3 non-CSCs, and (G) HEYA8 CSCs was measured using MTT assay after treatment with indicated solutions. (H) Normal human OSEs were transfected with 20 nM p70^S6K siRNA/G₆ or nonspecific (NS) siRNA/G₆ dendriplexes for the indicated time. p70^S6K protein levels were measured using western blot and quantified with β-actin as the control. Cell viability was measured using MTT assay. Data are presented as mean ± SD. *p < 0.05 versus NS siRNA/G₆.