Fig. 4.

Expression of Id3 and Runx2 was increased in arterioles in tissue sections from patients with calciphylaxis. Arterioles from a representative calciphylaxis patient stained strongly for p-SMAD 1/5/9 (a, b); an adjacent section stained strongly for the presence of Id3 (c, d). Strong p-SMAD 1/5/9 staining was detected in tissue sections from a second patient with calciphylaxis (e, f); an adjacent section stained strongly for the presence of Runx2 (g, h). Black arrows in (f, h) indicate the location of representative, positively-staining cells. Black boxes in (a, c, e, g) indicate the magnified regions shown in (b, d, f, h) respectively. Black bars in (a, c, e, g) indicate 100 μm. Black bars in (b, d, f, h) indicate 20 μm.