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. Author manuscript; available in PMC: 2018 Jun 28.
Published in final edited form as: J Am Chem Soc. 2017 Jun 19;139(25):8547–8551. doi: 10.1021/jacs.7b02801

Figure 4.

Figure 4

Inhibition of active import to nucleus significantly reduces nuclear accumulation of boronate-tagged eGFP. (a) eGFP-BB delivery before and (b) after inhibition of importin α/β pathway. (c) eGFP-BB delivery after inhibition of all active import pathway by ATP depletion. (d) eGFP-B delivery before and (e) after inhibition of importin α/β pathway. (f) eGFP-B delivery after inhibition of all active import pathway by ATP depletion. Scale bars: 20 μm. (g) Quantitative analysis of increased fluorescence intensity of eGFP-BB and eGFP-B in the nucleus after delivery with or without pretreatment using six cells in each group.