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. Author manuscript; available in PMC: 2018 Jan 11.
Published in final edited form as: Cancer Lett. 2017 Oct 9;411:171–181. doi: 10.1016/j.canlet.2017.09.045

Fig. 7.

Fig. 7

Effect of 1,25(OH)2D on oxidative stress under PC knockdown. Stably transfected MCF10A-ras cells expressing Dox inducible shRNA targeting PC were treated with vehicle or 1,25(OH)2D (10 nM) for 96 h with a concurrent Dox (10 μg/mL) treatment for the last 72 h (A) PC mRNA expression was measured after 96 h of treatment using qRT-PCR and normalized to 18S. (B) PC protein expression was measured after 96 h of treatment. Values were normalized to β-tubulin and are expressed relative to vehicle Dox(−) treated cells. Representative blot images are provided. (C–E) ROS levels, NADPH/NADP+ and GSH/GSSG ratios were measured after 96 h of treatment and are expressed relative to vehicle Dox(−) treated group. (F&G) Total GSSG and GSH levels are normalized to protein content. Values are expressed as mean ± S.E.M. (*) Asterisk indicates statistical significance relative to vehicle treated cells. Bars with different letters are statistically significant (P < 0.05).