13C fractional enrichments of GLU C4 in the tumors. Calculation of a–e are from 13C NMR spectra of C4 GLU as described in the text (Materials and Methods). d and e correspond to the fractions that have [4,5-13C] and [3,4,5-13C] isotopomers respectively. a is the fraction that has no 13C. (b+c) together represents the contributions from the metabolism of [1,2-13C]acetate by peripheral organs to tumor GLU C4 13C pool. FE is equal to (b+c+d+e) and corresponds to fractional enrichment of GLU C4 in the tumor.