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. 2017 Oct 24;15(1):235–241. doi: 10.3892/etm.2017.5372

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Copyright: © Yuan et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 2. — Effects of cyclic stretch on the viability and apoptosis of CESCs. CESCs were subjected to a 20% stretch at a frequency of 1 Hz for 12, 24, 36 or 48 h. (A) The cell viability was assessed using a Cell Counting Kit-8 assay. (B) Apoptosis was detected by flow cytometry using an Annexin V/PI assay. Representative flow cytometric dot plots obtained after double-staining with Annexin V-FITC and PI are presented. (C) Apoptotic indices from (B). Static CESCs (0 h) were used as a control. *P<0.05 vs. control group. CESCs, cartilage endplate-derived stem cells; FITC, fluorescein isothiocyanate; PI, propidium iodide.