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. 2017 Oct 24;15(1):235–241. doi: 10.3892/etm.2017.5372

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Copyright: © Yuan et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 3. — Effect of stress on the activity of caspase-3 and the expression of apoptosis-associated proteins. CESCs were subjected to a 20% stretch at a frequency of 1 Hz for different durations (12, 24, 36 or 48 h). (A) Caspase-3 activity assay. (B) Western blot analysis was performed to analyze the expression of Bcl-2 and BNIP3 GAPDH was used as a loading control. Representative western blot images are presented. (C) Quantification of the BNIP3/Bcl-2 ratio from (B). Static CESCs (0 h) were used as a control. *P<0.05 vs. control group. CESCs, cartilage endplate-derived stem cells; Bcl-2, B-cell lymphoma 2; BNIP3, Bcl-2/adenovirus E1B 19 kDa interacting protein 3.