Fig. 5.

Regulatory network mediated by DLL4 in macrophage subsets. a QRTPCR analyses for Notch family members and signaling molecules. Macrophages (M0) were incubated with LPS + IFNγ (M1), IL-4 (M2) and with (+) or without (−) DLL4 for 24 h. Results shown are the means of triplicate experiments. The data were normalized using a housekeeping gene (β actin) and expressed as 2^-ΔΔCt using untreated M0 as reference. b Representative western blots for pro-apoptotic and effector molecules in M1 and M2 after polarization with LPS + IFNγ or IL-4 for 48 h and with or without immobilized DLL4