Table IV.
Raw copies of each gene quantified by quantitative polymerase chain reaction (qPCR).
| Sample ID | Replicate | Sample type | Technical replicate | 16S rRNA | sul1 | sul2 | tetA | tetB | tetM | tetO | tetQ | tetW | ermA | ermB | ermC | ermF | mefA | qnrS | oqxB | blaSHV | blaCTX-M | blaTEM1 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| A | 1 | Fecal | 1 | 135316848.00 | 20470.49 | 8431.89 | 2851.00 | 1350.12 | 471012.72 | 8856329.00 | 48037360.01 | 1674731.50 | 844.93 | 2187571.00 | 40.85a | 205467.56 | 10500624.00 | 50.30 | ||||
| 2 | 131714168.01 | 17365.96 | 7998.46 | 2735.85 | 1447.68 | 308166.19 | 9390889.00 | 49510743.99 | 1604023.00 | 684.48 | 1602458.00 | 217129.28 | 7118568.00 | 55.96 | ||||||||
| 3 | 129662864.01 | 18957.58 | 8862.46 | 2650.42 | 1514.21 | 267133.56 | 9903522.00 | 51098516.02 | 1701210.50 | 974.48 | 1671816.50 | 33.60a | 211299.39 | 5797324.00 | ||||||||
| A | 2 | Fecal | 1 | 99899663.99 | 21102.22 | 40312.38 | 8108.91 | 439.11 | 441146.84 | 6130922.00 | 40700640.01 | 1327763.50 | 4753.59 | 1276430.12 | 118.17 | 116988.45 | 3365209.25 | 86.74 | ||||
| 2 | 103062087.99 | 18706.49 | 51132.17 | 7737.51 | 378.95 | 423588.78 | 5981603.50 | 39793516.00 | 1322640.25 | 5150.98 | 1038140.06 | 130.73 | 117221.91 | 3204971.25 | ||||||||
| 3 | 100093440.01 | 21806.00 | 43241.84 | 8301.56 | 315.31 | 360798.62 | 6061510.50 | 38425511.99 | 1265414.38 | 5476.85 | 1235953.88 | 122629.25 | 3724736.50 | 81.03 | ||||||||
| A | 3 | Fecal | 1 | 111629152.01 | 25474.63 | 22078.60 | 5601.00 | 7009.02 | 584707.06 | 6507431.00 | 53670336.00 | 1515935.88 | 4014.69 | 2352380.00 | 1708.55 | 336977.94 | 3663204.25 | 236.53 | ||||
| 2 | 122443199.99 | 28652.35 | 22681.12 | 5607.93 | 6733.56 | 543316.44 | 6483575.50 | 53856492.00 | 1373533.13 | 3135.03 | 2622462.25 | 1310.36 | 327080.19 | 3506120.00 | 287.97 | |||||||
| 3 | 119955575.99 | 31465.53 | 22883.97 | 6646.54 | 7191.74 | 426515.75 | 6950969.00 | 58135256.00 | 1445576.13 | 3889.44 | 2624452.75 | 991.10 | 348714.59 | 4450952.00 | 398.74 | |||||||
| B | 1 | Fecal | 1 | 159316384.01 | 111101.95 | 165836.91 | 6728.63 | 12306.90 | 282245.50 | 10369626.00 | 61502276.00 | 2224887.75 | 117338.26 | 900.45 | 282343.72 | 9378331.00 | 2818.12 | |||||
| 2 | 146688688.00 | 113397.43 | 118706.06 | 6479.08 | 12935.85 | 239815.77 | 9428455.00 | 59709327.99 | 2088906.13 | 110939.27 | 2213242.00 | 268473.81 | 8159826.50 | 2728.29 | ||||||||
| 3 | 161305823.98 | 116997.45 | 156138.98 | 6362.95 | 13036.56 | 10377423.00 | 68864232.01 | 2255860.75 | 117087.73 | 2805674.50 | 473.71 | 279472.25 | 6569808.50 | 3095.71 | ||||||||
| B | 2 | Fecal | 1 | 81552063.99 | 98154.14 | 110756.03 | 5701.19 | 59988.39 | 302924.81 | 6453254.00 | 33777476.01 | 1422558.87 | 73125.31 | 2202396.75 | 1074.36 | 168106.72 | 4872355.50 | |||||
| 2 | 85743295.99 | 97176.06 | 79365.62 | 5726.55 | 55252.45 | 242925.16 | 6351378.00 | 35357060.00 | 1371462.75 | 50762.96 | 2299559.75 | 177602.81 | 4400084.00 | 81.93 | ||||||||
| 3 | 79841408.01 | 80799.32 | 4670.81 | 50258.24 | 5654909.00 | 26986128.00 | 1183911.13 | 1886979.50 | 1082.50 | 152050.31 | 3529317.50 | 92.84 | ||||||||||
| B | 3 | Fecal | 1 | 183421056.01 | 47936.98 | 31062.49 | 19571.46 | 11931.66 | 65516.30 | 13083021.00 | 48467588.00 | 3201282.50 | 25943.95 | 8699972.00 | 1731.31 | 499035.00 | 9397475.00 | 91.60 | 880.61 | |||
| 2 | 174064223.98 | 44109.56 | 33776.75 | 19191.80 | 12006.78 | 91600.15 | 13306456.00 | 54075300.00 | 3315827.25 | 25552.25 | 9034571.00 | 1872.85 | 477091.50 | 12886706.00 | 105.42 | 796.97 | ||||||
| 3 | 194329407.99 | 46224.36 | 33637.20 | 21135.25 | 13638.56 | 105886.27 | 12358680.00 | 61737384.00 | 3250019.00 | 24779.23 | 8225782.50 | 1463.09 | 391836.84 | 10886674.00 | 783.58 | |||||||
| C | 1 | Fecal | 1 | 132025615.99 | 59021.54 | 198582.59 | 3159.40 | 557.26 | 1125759.62 | 7817069.50 | 49755600.00 | 1568247.75 | 30813.44 | 418717.34 | 363393.00 | 3167658.75 | 37.20a | |||||
| 2 | 131376103.98 | 59779.86 | 184612.44 | 3836.21 | 523.14 | 1045928.12 | 7809439.00 | 53673215.99 | 1574292.50 | 33599.78 | 482782.94 | 110.83 | 423221.34 | 2862075.50 | 22.55a | |||||||
| 3 | 120998464.00 | 56464.17 | 168604.11 | 3454.13 | 377.25 | 829512.44 | 7937674.50 | 46971811.99 | 1528236.12 | 32392.02 | 424963.19 | 72.78 | 373501.34 | 3173973.50 | 24.64a | |||||||
| C | 2 | Fecal | 1 | 122397007.99 | 72122.42 | 235897.00 | 13834.71 | 1891.63 | 1006640.69 | 6830077.00 | 60875284.02 | 1388427.00 | 30666.98 | 547956.31 | 19.39a | 687731.19 | 2188730.00 | 15.58a | 2384.82 | |||
| 2 | 122793696.00 | 66459.59 | 220050.12 | 13869.91 | 1745.99 | 950735.63 | 7544167.50 | 57674784.00 | 1519405.00 | 30872.13 | 566380.69 | 23.10a | 693207.62 | 2100251.50 | 17.83a | 2385.01 | ||||||
| 3 | 115632415.99 | 63934.21 | 163830.08 | 12835.12 | 2014.22 | 1051637.88 | 6819442.00 | 57393216.01 | 1415696.38 | 30387.80 | 501701.44 | 16.31a | 685353.69 | 2969424.25 | 2114.98 | |||||||
| C | 3 | Fecal | 1 | 137840176.00 | 46671.25 | 179057.03 | 18671.75 | 5545.93 | 113185.48 | 6309339.00 | 28700536.00 | 1571610.50 | 10945.18 | 1911604.63 | 650.14 | 130912.76 | 4955301.00 | 234.94 | ||||
| 2 | 145724783.99 | 44179.83 | 97156.34 | 17989.21 | 5403.88 | 100214.77 | 6724203.50 | 29734530.00 | 1668433.50 | 2173519.50 | 716.32 | 143671.91 | 5677877.00 | 281.04 | ||||||||
| 3 | 135602272.01 | 43281.54 | 130144.59 | 17046.96 | 5018.45 | 119425.29 | 6746098.00 | 28995622.00 | 1542394.00 | 8665.21 | 2137903.00 | 140682.22 | 5617090.50 | 220.93 | ||||||||
| DC | 1 | Fecal | 1 | 129925072.00 | 40353.48 | 373749.06 | 4017.37 | 823.61 | 168191.22 | 8200801.00 | 49411755.99 | 1973424.25 | 74038.43 | 11354147.00 | 6525.17 | 352319.09 | 9235876.00 | 295.75 | ||||
| 2 | 115599064.00 | 31477.65 | 429841.19 | 3568.97 | 608.41 | 165141.44 | 7787328.00 | 46216495.99 | 1773117.37 | 80707.81 | 9423764.00 | 6217.44 | 320004.37 | 7344657.50 | 288.50 | |||||||
| 3 | 118867728.01 | 38710.29 | 373662.66 | 1050.50 | 171959.63 | 7115402.00 | 45508143.99 | 1694098.62 | 84501.50 | 5095.33 | 305576.59 | 4809984.50 | 274.63 | |||||||||
| DC | 2 | Fecal | 1 | 129377624.00 | 22421.61 | 79879.81 | 2969.62 | 200728.61 | 8610279.00 | 43357992.01 | 1950964.50 | 13844.17 | 377.58 | 203484.56 | 8058277.00 | 466.72 | ||||||
| 2 | 141131536.00 | 25813.30 | 78503.76 | 3243.03 | 570.82 | 192892.69 | 9469729.00 | 50623836.01 | 1975484.00 | 14494.78 | 5714419.50 | 190509.19 | 8697406.00 | 499.05 | ||||||||
| 3 | 145017184.01 | 22801.69 | 76488.68 | 3596.93 | 669.44 | 147117.75 | 8740696.00 | 51748652.01 | 1996960.63 | 12459.60 | 5409304.00 | 408.99 | 218296.69 | 6174802.00 | 503.26 | |||||||
| DC | 3 | Fecal | 1 | 43101751.99 | 10237.82 | 13785.80 | 1305.03 | 517.25 | 111468.91 | 3666688.00 | 18258204.00 | 642751.06 | 17556.67 | 529002.56 | 3348.62 | 48692.04 | 1979577.50 | 13.06a | 100.59 | |||
| 2 | 41444508.01 | 12869.65 | 13924.53 | 1194.60 | 389.30 | 110159.65 | 3717029.25 | 17124558.00 | 604603.62 | 18156.68 | 566633.19 | 3043.43 | 43779.34 | 2204189.50 | 12.00a | 66.44 | ||||||
| 3 | 44755803.99 | 8382.83 | 13732.72 | 1946.21 | 407.02 | 123459.26 | 3919679.75 | 20919260.00 | 666029.56 | 17895.37 | 580689.13 | 4296.82 | 49713.02 | 2199199.50 | 20.35a | 77.79 | ||||||
| DN | 1 | Fecal | 1 | 37480983.99 | 12179.08 | 5156.01 | 241.47 | 12121.73 | 2141243.00 | 9101782.00 | 412804.25 | 2945.59 | 575923.06 | 119.52 | 26330.30 | 1817781.12 | 53.34 | |||||
| 2 | 36215332.00 | 6770.08 | 5549.21 | 227.09 | 10885.59 | 2091591.50 | 8376621.00 | 421088.66 | 3005.33 | 459052.22 | 24433.53 | 1735015.75 | 39.39a | |||||||||
| 3 | 37729760.00 | 7409.72 | 5326.78 | 316.66 | 270.52 | 12177.91 | 2101451.50 | 8117923.50 | 415199.91 | 2729.59 | 400774.44 | 120.27 | 23920.72 | 1349194.50 | ||||||||
| DN | 2 | Fecal | 1 | 44521535.99 | 16224.93 | 19464.00 | 371.90 | 217.13 | 55777.77 | 1691142.12 | 9644993.00 | 334087.06 | 7747.75 | 71843.28 | 70.28 | 18048.39 | 1409157.62 | 181.48 | ||||
| 2 | 43508420.00 | 12930.62 | 19531.71 | 376.52 | 309.44 | 40239.45 | 1635285.25 | 9207957.00 | 308672.87 | 7507.85 | 64030.17 | 16776.38 | 1433570.25 | |||||||||
| 3 | 46520511.99 | 19602.84 | 411.49 | 370.36 | 43530.51 | 1518456.75 | 9917966.00 | 301118.59 | 7760.27 | 70832.16 | 60.59 | 18279.78 | 219.55 | |||||||||
| DN | 3 | Fecal | 1 | 141902400.00 | 57814.70 | 310189.19 | 102429.73 | 25710.93 | 440572.31 | 6990363.50 | 47887328.01 | 2384895.00 | 50948.66 | 1549203.12 | 203043.66 | 6134732.50 | 446.82 | |||||
| 2 | 143139519.99 | 56497.85 | 301551.94 | 102248.34 | 23645.28 | 291791.06 | 6489048.00 | 51405652.01 | 2096934.12 | 53354.60 | 1735329.00 | 188.29 | 211177.77 | 9015095.00 | 594.74 | |||||||
| 3 | 143673359.99 | 62237.89 | 277239.03 | 115880.05 | 26122.71 | 335872.87 | 7051654.50 | 1673693.75 | 58052.32 | 1695864.37 | 216.24 | 221886.25 | 6697095.50 | 580.79 | ||||||||
| CB | 1 | Catch Basin | 1 | 18703190.00 | 146279.45 | 536011.19 | 398.38 | 59815.21 | 14823.55 | 7636.33 | 11995.24 | 106602.04 | 971.07 | 3513.79 | 10157.67 | 14.28a | ||||||
| 2 | 16186165.00 | 161438.92 | 468081.38 | 494.18 | 111.21 | 45805.99 | 15114.80 | 347608.06 | 6991.99 | 12288.96 | 109660.18 | 3364.80 | 7974.03 | 18.20a | ||||||||
| 3 | 18902718.00 | 155824.48 | 548441.31 | 450.63 | 140.99 | 57605.87 | 16031.42 | 409233.78 | 7820.98 | 12553.37 | 97607.95 | 856.06 | 3706.70 | 9697.13 | ||||||||
| CB | 2 | Catch Basin | 1 | 15063999.00 | 379057.69 | 414152.28 | 153.42 | 113603.36 | 25949.88 | 604500.81 | 17091.27 | 37464.08 | 86781.61 | 2903.46 | 11392.22 | 27065.91 | 16.38a | |||||
| 2 | 14237252.00 | 348791.00 | 420699.09 | 241.13 | 161.34 | 114591.84 | 24069.18 | 589924.69 | 14613.12 | 32663.29 | 90019.25 | 2947.21 | 9539.64 | 25740.40 | 11.30a | 18.42a | ||||||
| 3 | 16192147.00 | 390327.22 | 443561.25 | 328.65 | 113.33 | 122569.90 | 24550.94 | 664899.37 | 16414.96 | 32869.02 | 86747.30 | 2572.93 | 11765.55 | 29211.59 | 18.65a | |||||||
| CB | 3 | Catch Basin | 1 | 15566558.00 | 152165.77 | 156909.59 | 2241.81 | 7231.61 | 24904.78 | 175294.53 | 889.47 | 28864.72 | 15912.83 | 21920.78 | ||||||||
| 2 | 14022131.00 | 144100.55 | 112549.97 | 2045.47 | 8010.50 | 24839.63 | 170369.31 | 882.64 | 23696.39 | 14753.91 | 20901.29 | |||||||||||
| 3 | 15329532.00 | 151966.83 | 115317.68 | 1877.90 | 23883.96 | 187182.70 | 764.37 | 28468.64 | 16060.86 | 22305.12 | ||||||||||||
| CB | 4 | Catch Basin | 1 | 18983356.00 | 43344.68 | 381580.47 | 30048.64 | 886.17 | 95362.10 | 41749.20 | 845609.25 | 23801.66 | 74629.36 | 872866.75 | 4966.11 | 27366.06 | 47067.50 | 17.32a | 92.56 | |||
| 2 | 19714306.00 | 48460.45 | 395349.41 | 29622.58 | 979.91 | 100002.84 | 40699.20 | 868928.13 | 25048.52 | 79670.13 | 877625.31 | 5355.82 | 29033.96 | 50241.57 | 21.18a | 137.01 | ||||||
| 3 | 19069442.00 | 52214.59 | 382200.75 | 29031.36 | 812.59 | 99042.60 | 40327.45 | 874559.63 | 25561.80 | 79933.93 | 830863.19 | 4543.10 | 32221.33 | 54585.04 | 113.54 | |||||||
| CB | 5 | Catch Basin | 1 | 32513026.00 | 113575.21 | 200583.11 | 1063.29 | 552.15 | 137919.02 | 40425.26 | 1029176.44 | 43591.25 | 378695.41 | 2924847.50 | 26179.17 | 181086.98 | 70543.54 | 10.36a | 89.32 | |||
| 2 | 27277818.00 | 99679.20 | 209723.22 | 951.40 | 623.87 | 140190.69 | 41385.32 | 863914.69 | 39857.52 | 301237.62 | 2567297.75 | 21340.53 | 164171.45 | 59324.51 | 14.22a | 123.25 | ||||||
| 3 | 37447248.01 | 94858.66 | 211840.95 | 865.69 | 499.67 | 122132.66 | 35514.40 | 37923.13 | 279235.09 | 2484248.25 | 27479.65 | 162058.97 | 54576.43 | 13.50a | 96.00 | |||||||
| Influent | 1 | Sewage | 1 | 82388256.01 | 995124.75 | 187163.92 | 32838.39 | 6655.73 | 20719.75 | 387385.13 | 2711903.50 | 68745.53 | 1029.51 | 19532302.00 | 526.72 | 284718.87 | 83297.43 | 1309367.00 | 119.69 | 3109.30 | 1336.238403 | 65035.35 |
| Influent | 2 | 81109407.99 | 182969.22 | 33881.34 | 7084.83 | 22775.30 | 375026.16 | 2652150.75 | 62506.80 | 1220.07 | 18525322.00 | 283778.12 | 96386.62 | 1388566.87 | 119.31 | 3181.14 | 1412.349731 | 67611.09 | ||||
| 3 | 70840319.99 | 1072630.87 | 198985.41 | 32978.54 | 7330.73 | 21845.22 | 391636.34 | 2956557.75 | 79212.13 | 1418.44 | 20022754.00 | 683.35 | 279162.63 | 109074.01 | 1444878.25 | 124.47 | 3303.45 | 1588.18042 | 79525.66 | |||
| Influent | 2 | Sewage | 1 | 37155672.00 | 750581.50 | 115719.91 | 11906.55 | 2580.00 | 5449.93 | 150048.62 | 742904.75 | 23979.22 | 4121.26 | 8816599.00 | 597.24 | 109423.16 | 60308.86 | 1441790.87 | 87.47 | 2162.38 | 385.20 | 12393.67 |
| Influent | 2 | 37180852.00 | 716767.50 | 85876.12 | 12473.78 | 2750.65 | 6349.06 | 148901.39 | 699771.12 | 23660.55 | 3634.77 | 8749802.00 | 519.54 | 103746.18 | 56488.30 | 1472774.50 | 83.56 | 2472.96 | 389.61 | 11638.84 | ||
| 3 | 39584727.99 | 84036.18 | 13184.17 | 2932.60 | 5946.53 | 157894.20 | 793511.44 | 28515.30 | 4517.93 | 8972806.00 | 531.34 | 103627.99 | 61474.34 | 1560031.25 | 98.00 | 2225.99 | 346.83 | 13289.37 | ||||
| Effluent | 1 | Sewage | 1 | 4804898.00 | 89705.76 | 15962.68 | 945.28 | 9.00a | 349.75 | 4498.64 | 18368.64 | 1065.17 | 25.76a | 259306.09 | 375.98 | 3468.21 | 1561.33 | 7430.90 | 0.67a | 14.84a | 84.91 | |
| Effluent | 2 | 4818336.50 | 90243.05 | 17754.80 | 884.22 | 12.52a | 407.76 | 4331.12 | 17219.56 | 946.12 | 19.65a | 263796.94 | 417.42 | 3313.91 | 1714.50 | 7552.45 | 0.99a | 86.03 | 5.37a | |||
| 3 | 5012697.00 | 94629.18 | 17782.24 | 937.30 | 423.61 | 4921.21 | 17450.86 | 1169.20 | 279994.22 | 2545.83 | 1627.88 | 7556.24 | 0.63a | 21.92a | 77.70 | 5.98a | ||||||
| Effluent | 2 | Sewage | 1 | 2654580.00 | 52085.63 | 34527.16 | 719.34 | 140.03 | 182.84 | 3215.77 | 11869.75 | 643.48 | 98357.73 | 7390.41 | 1079.39 | 20827.89 | 0.63a | 30.92a | 12.09a | 118.66 | ||
| Effluent | 2 | 2738544.25 | 51803.52 | 36214.01 | 835.13 | 205.53 | 146.19 | 3144.79 | 13364.86 | 600.82 | 31.64a | 79695.05 | 74.09 | 6933.53 | 870.97 | 22355.65 | 1.02a | 116.86 | ||||
| 3 | 2343376.75 | 48166.34 | 32328.57 | 867.35 | 238.85 | 160.51 | 2449.20 | 11197.99 | 410.11 | 32.88a | 75651.98 | 28.49a | 6285.76 | 867.81 | 18702.66 | 31.38a | 7.56a | 143.13 | ||||
| Creek | 1 | Ephermal | 1 | 2768154.25 | 1015.00 | 10.07a | 209.42 | |||||||||||||||
| Creek | 2 | 2925811.00 | 1217.49 | 24.64a | 57.91 | 484.38 | 583.61a | 186.39 | 20.95a | 8.84a | ||||||||||||
| 3 | 2850461.25 | 1026.70 | 20.21a | 51.28 | 471.48 | 8.23a | 41.15a | 22.32a | 5.27a | |||||||||||||
| Creek | 2 | Ephermal | 1 | 8306417.50 | 6438.36 | 455.97 | 30.55a | 297.50 | 1736.26 | 499.33 | 680.35a | 189.26 | 36.38a | |||||||||
| Creek | 2 | 8646568.00 | 5641.77 | 484.49 | 237.73 | 1971.78 | 649.63 | 45.14a | 196.52 | |||||||||||||
| 3 | 8265799.50 | 6121.54 | 374.68 | 50.21a | 234.69 | 2211.98 | 558.36 | 218.96 | 31.16a |
a
Values outside standard curve range. Absence of a value indicates that the resistance determinant was not detected.