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. 2018 Jan;82(1):24–38.

Table IV.

Raw copies of each gene quantified by quantitative polymerase chain reaction (qPCR).

Sample ID Replicate Sample type Technical replicate 16S rRNA sul1 sul2 tetA tetB tetM tetO tetQ tetW ermA ermB ermC ermF mefA qnrS oqxB blaSHV blaCTX-M blaTEM1
A 1 Fecal 1 135316848.00 20470.49 8431.89 2851.00 1350.12 471012.72 8856329.00 48037360.01 1674731.50 844.93 2187571.00 40.85a 205467.56 10500624.00 50.30
2 131714168.01 17365.96 7998.46 2735.85 1447.68 308166.19 9390889.00 49510743.99 1604023.00 684.48 1602458.00 217129.28 7118568.00 55.96
3 129662864.01 18957.58 8862.46 2650.42 1514.21 267133.56 9903522.00 51098516.02 1701210.50 974.48 1671816.50 33.60a 211299.39 5797324.00
A 2 Fecal 1 99899663.99 21102.22 40312.38 8108.91 439.11 441146.84 6130922.00 40700640.01 1327763.50 4753.59 1276430.12 118.17 116988.45 3365209.25 86.74
2 103062087.99 18706.49 51132.17 7737.51 378.95 423588.78 5981603.50 39793516.00 1322640.25 5150.98 1038140.06 130.73 117221.91 3204971.25
3 100093440.01 21806.00 43241.84 8301.56 315.31 360798.62 6061510.50 38425511.99 1265414.38 5476.85 1235953.88 122629.25 3724736.50 81.03
A 3 Fecal 1 111629152.01 25474.63 22078.60 5601.00 7009.02 584707.06 6507431.00 53670336.00 1515935.88 4014.69 2352380.00 1708.55 336977.94 3663204.25 236.53
2 122443199.99 28652.35 22681.12 5607.93 6733.56 543316.44 6483575.50 53856492.00 1373533.13 3135.03 2622462.25 1310.36 327080.19 3506120.00 287.97
3 119955575.99 31465.53 22883.97 6646.54 7191.74 426515.75 6950969.00 58135256.00 1445576.13 3889.44 2624452.75 991.10 348714.59 4450952.00 398.74
B 1 Fecal 1 159316384.01 111101.95 165836.91 6728.63 12306.90 282245.50 10369626.00 61502276.00 2224887.75 117338.26 900.45 282343.72 9378331.00 2818.12
2 146688688.00 113397.43 118706.06 6479.08 12935.85 239815.77 9428455.00 59709327.99 2088906.13 110939.27 2213242.00 268473.81 8159826.50 2728.29
3 161305823.98 116997.45 156138.98 6362.95 13036.56 10377423.00 68864232.01 2255860.75 117087.73 2805674.50 473.71 279472.25 6569808.50 3095.71
B 2 Fecal 1 81552063.99 98154.14 110756.03 5701.19 59988.39 302924.81 6453254.00 33777476.01 1422558.87 73125.31 2202396.75 1074.36 168106.72 4872355.50
2 85743295.99 97176.06 79365.62 5726.55 55252.45 242925.16 6351378.00 35357060.00 1371462.75 50762.96 2299559.75 177602.81 4400084.00 81.93
3 79841408.01 80799.32 4670.81 50258.24 5654909.00 26986128.00 1183911.13 1886979.50 1082.50 152050.31 3529317.50 92.84
B 3 Fecal 1 183421056.01 47936.98 31062.49 19571.46 11931.66 65516.30 13083021.00 48467588.00 3201282.50 25943.95 8699972.00 1731.31 499035.00 9397475.00 91.60 880.61
2 174064223.98 44109.56 33776.75 19191.80 12006.78 91600.15 13306456.00 54075300.00 3315827.25 25552.25 9034571.00 1872.85 477091.50 12886706.00 105.42 796.97
3 194329407.99 46224.36 33637.20 21135.25 13638.56 105886.27 12358680.00 61737384.00 3250019.00 24779.23 8225782.50 1463.09 391836.84 10886674.00 783.58
C 1 Fecal 1 132025615.99 59021.54 198582.59 3159.40 557.26 1125759.62 7817069.50 49755600.00 1568247.75 30813.44 418717.34 363393.00 3167658.75 37.20a
2 131376103.98 59779.86 184612.44 3836.21 523.14 1045928.12 7809439.00 53673215.99 1574292.50 33599.78 482782.94 110.83 423221.34 2862075.50 22.55a
3 120998464.00 56464.17 168604.11 3454.13 377.25 829512.44 7937674.50 46971811.99 1528236.12 32392.02 424963.19 72.78 373501.34 3173973.50 24.64a
C 2 Fecal 1 122397007.99 72122.42 235897.00 13834.71 1891.63 1006640.69 6830077.00 60875284.02 1388427.00 30666.98 547956.31 19.39a 687731.19 2188730.00 15.58a 2384.82
2 122793696.00 66459.59 220050.12 13869.91 1745.99 950735.63 7544167.50 57674784.00 1519405.00 30872.13 566380.69 23.10a 693207.62 2100251.50 17.83a 2385.01
3 115632415.99 63934.21 163830.08 12835.12 2014.22 1051637.88 6819442.00 57393216.01 1415696.38 30387.80 501701.44 16.31a 685353.69 2969424.25 2114.98
C 3 Fecal 1 137840176.00 46671.25 179057.03 18671.75 5545.93 113185.48 6309339.00 28700536.00 1571610.50 10945.18 1911604.63 650.14 130912.76 4955301.00 234.94
2 145724783.99 44179.83 97156.34 17989.21 5403.88 100214.77 6724203.50 29734530.00 1668433.50 2173519.50 716.32 143671.91 5677877.00 281.04
3 135602272.01 43281.54 130144.59 17046.96 5018.45 119425.29 6746098.00 28995622.00 1542394.00 8665.21 2137903.00 140682.22 5617090.50 220.93
DC 1 Fecal 1 129925072.00 40353.48 373749.06 4017.37 823.61 168191.22 8200801.00 49411755.99 1973424.25 74038.43 11354147.00 6525.17 352319.09 9235876.00 295.75
2 115599064.00 31477.65 429841.19 3568.97 608.41 165141.44 7787328.00 46216495.99 1773117.37 80707.81 9423764.00 6217.44 320004.37 7344657.50 288.50
3 118867728.01 38710.29 373662.66 1050.50 171959.63 7115402.00 45508143.99 1694098.62 84501.50 5095.33 305576.59 4809984.50 274.63
DC 2 Fecal 1 129377624.00 22421.61 79879.81 2969.62 200728.61 8610279.00 43357992.01 1950964.50 13844.17 377.58 203484.56 8058277.00 466.72
2 141131536.00 25813.30 78503.76 3243.03 570.82 192892.69 9469729.00 50623836.01 1975484.00 14494.78 5714419.50 190509.19 8697406.00 499.05
3 145017184.01 22801.69 76488.68 3596.93 669.44 147117.75 8740696.00 51748652.01 1996960.63 12459.60 5409304.00 408.99 218296.69 6174802.00 503.26
DC 3 Fecal 1 43101751.99 10237.82 13785.80 1305.03 517.25 111468.91 3666688.00 18258204.00 642751.06 17556.67 529002.56 3348.62 48692.04 1979577.50 13.06a 100.59
2 41444508.01 12869.65 13924.53 1194.60 389.30 110159.65 3717029.25 17124558.00 604603.62 18156.68 566633.19 3043.43 43779.34 2204189.50 12.00a 66.44
3 44755803.99 8382.83 13732.72 1946.21 407.02 123459.26 3919679.75 20919260.00 666029.56 17895.37 580689.13 4296.82 49713.02 2199199.50 20.35a 77.79
DN 1 Fecal 1 37480983.99 12179.08 5156.01 241.47 12121.73 2141243.00 9101782.00 412804.25 2945.59 575923.06 119.52 26330.30 1817781.12 53.34
2 36215332.00 6770.08 5549.21 227.09 10885.59 2091591.50 8376621.00 421088.66 3005.33 459052.22 24433.53 1735015.75 39.39a
3 37729760.00 7409.72 5326.78 316.66 270.52 12177.91 2101451.50 8117923.50 415199.91 2729.59 400774.44 120.27 23920.72 1349194.50
DN 2 Fecal 1 44521535.99 16224.93 19464.00 371.90 217.13 55777.77 1691142.12 9644993.00 334087.06 7747.75 71843.28 70.28 18048.39 1409157.62 181.48
2 43508420.00 12930.62 19531.71 376.52 309.44 40239.45 1635285.25 9207957.00 308672.87 7507.85 64030.17 16776.38 1433570.25
3 46520511.99 19602.84 411.49 370.36 43530.51 1518456.75 9917966.00 301118.59 7760.27 70832.16 60.59 18279.78 219.55
DN 3 Fecal 1 141902400.00 57814.70 310189.19 102429.73 25710.93 440572.31 6990363.50 47887328.01 2384895.00 50948.66 1549203.12 203043.66 6134732.50 446.82
2 143139519.99 56497.85 301551.94 102248.34 23645.28 291791.06 6489048.00 51405652.01 2096934.12 53354.60 1735329.00 188.29 211177.77 9015095.00 594.74
3 143673359.99 62237.89 277239.03 115880.05 26122.71 335872.87 7051654.50 1673693.75 58052.32 1695864.37 216.24 221886.25 6697095.50 580.79
CB 1 Catch Basin 1 18703190.00 146279.45 536011.19 398.38 59815.21 14823.55 7636.33 11995.24 106602.04 971.07 3513.79 10157.67 14.28a
2 16186165.00 161438.92 468081.38 494.18 111.21 45805.99 15114.80 347608.06 6991.99 12288.96 109660.18 3364.80 7974.03 18.20a
3 18902718.00 155824.48 548441.31 450.63 140.99 57605.87 16031.42 409233.78 7820.98 12553.37 97607.95 856.06 3706.70 9697.13
CB 2 Catch Basin 1 15063999.00 379057.69 414152.28 153.42 113603.36 25949.88 604500.81 17091.27 37464.08 86781.61 2903.46 11392.22 27065.91 16.38a
2 14237252.00 348791.00 420699.09 241.13 161.34 114591.84 24069.18 589924.69 14613.12 32663.29 90019.25 2947.21 9539.64 25740.40 11.30a 18.42a
3 16192147.00 390327.22 443561.25 328.65 113.33 122569.90 24550.94 664899.37 16414.96 32869.02 86747.30 2572.93 11765.55 29211.59 18.65a
CB 3 Catch Basin 1 15566558.00 152165.77 156909.59 2241.81 7231.61 24904.78 175294.53 889.47 28864.72 15912.83 21920.78
2 14022131.00 144100.55 112549.97 2045.47 8010.50 24839.63 170369.31 882.64 23696.39 14753.91 20901.29
3 15329532.00 151966.83 115317.68 1877.90 23883.96 187182.70 764.37 28468.64 16060.86 22305.12
CB 4 Catch Basin 1 18983356.00 43344.68 381580.47 30048.64 886.17 95362.10 41749.20 845609.25 23801.66 74629.36 872866.75 4966.11 27366.06 47067.50 17.32a 92.56
2 19714306.00 48460.45 395349.41 29622.58 979.91 100002.84 40699.20 868928.13 25048.52 79670.13 877625.31 5355.82 29033.96 50241.57 21.18a 137.01
3 19069442.00 52214.59 382200.75 29031.36 812.59 99042.60 40327.45 874559.63 25561.80 79933.93 830863.19 4543.10 32221.33 54585.04 113.54
CB 5 Catch Basin 1 32513026.00 113575.21 200583.11 1063.29 552.15 137919.02 40425.26 1029176.44 43591.25 378695.41 2924847.50 26179.17 181086.98 70543.54 10.36a 89.32
2 27277818.00 99679.20 209723.22 951.40 623.87 140190.69 41385.32 863914.69 39857.52 301237.62 2567297.75 21340.53 164171.45 59324.51 14.22a 123.25
3 37447248.01 94858.66 211840.95 865.69 499.67 122132.66 35514.40 37923.13 279235.09 2484248.25 27479.65 162058.97 54576.43 13.50a 96.00
Influent 1 Sewage 1 82388256.01 995124.75 187163.92 32838.39 6655.73 20719.75 387385.13 2711903.50 68745.53 1029.51 19532302.00 526.72 284718.87 83297.43 1309367.00 119.69 3109.30 1336.238403 65035.35
Influent 2 81109407.99 182969.22 33881.34 7084.83 22775.30 375026.16 2652150.75 62506.80 1220.07 18525322.00 283778.12 96386.62 1388566.87 119.31 3181.14 1412.349731 67611.09
3 70840319.99 1072630.87 198985.41 32978.54 7330.73 21845.22 391636.34 2956557.75 79212.13 1418.44 20022754.00 683.35 279162.63 109074.01 1444878.25 124.47 3303.45 1588.18042 79525.66
Influent 2 Sewage 1 37155672.00 750581.50 115719.91 11906.55 2580.00 5449.93 150048.62 742904.75 23979.22 4121.26 8816599.00 597.24 109423.16 60308.86 1441790.87 87.47 2162.38 385.20 12393.67
Influent 2 37180852.00 716767.50 85876.12 12473.78 2750.65 6349.06 148901.39 699771.12 23660.55 3634.77 8749802.00 519.54 103746.18 56488.30 1472774.50 83.56 2472.96 389.61 11638.84
3 39584727.99 84036.18 13184.17 2932.60 5946.53 157894.20 793511.44 28515.30 4517.93 8972806.00 531.34 103627.99 61474.34 1560031.25 98.00 2225.99 346.83 13289.37
Effluent 1 Sewage 1 4804898.00 89705.76 15962.68 945.28 9.00a 349.75 4498.64 18368.64 1065.17 25.76a 259306.09 375.98 3468.21 1561.33 7430.90 0.67a 14.84a 84.91
Effluent 2 4818336.50 90243.05 17754.80 884.22 12.52a 407.76 4331.12 17219.56 946.12 19.65a 263796.94 417.42 3313.91 1714.50 7552.45 0.99a 86.03 5.37a
3 5012697.00 94629.18 17782.24 937.30 423.61 4921.21 17450.86 1169.20 279994.22 2545.83 1627.88 7556.24 0.63a 21.92a 77.70 5.98a
Effluent 2 Sewage 1 2654580.00 52085.63 34527.16 719.34 140.03 182.84 3215.77 11869.75 643.48 98357.73 7390.41 1079.39 20827.89 0.63a 30.92a 12.09a 118.66
Effluent 2 2738544.25 51803.52 36214.01 835.13 205.53 146.19 3144.79 13364.86 600.82 31.64a 79695.05 74.09 6933.53 870.97 22355.65 1.02a 116.86
3 2343376.75 48166.34 32328.57 867.35 238.85 160.51 2449.20 11197.99 410.11 32.88a 75651.98 28.49a 6285.76 867.81 18702.66 31.38a 7.56a 143.13
Creek 1 Ephermal 1 2768154.25 1015.00 10.07a 209.42
Creek 2 2925811.00 1217.49 24.64a 57.91 484.38 583.61a 186.39 20.95a 8.84a
3 2850461.25 1026.70 20.21a 51.28 471.48 8.23a 41.15a 22.32a 5.27a
Creek 2 Ephermal 1 8306417.50 6438.36 455.97 30.55a 297.50 1736.26 499.33 680.35a 189.26 36.38a
Creek 2 8646568.00 5641.77 484.49 237.73 1971.78 649.63 45.14a 196.52
3 8265799.50 6121.54 374.68 50.21a 234.69 2211.98 558.36 218.96 31.16a
a

Values outside standard curve range. Absence of a value indicates that the resistance determinant was not detected.