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. Author manuscript; available in PMC: 2019 Jan 8.
Published in final edited form as: Dev Cell. 2018 Jan 8;44(1):73–86.e4. doi: 10.1016/j.devcel.2017.12.011

Figure 4. Large Hydrophobic Residues Are Crucial for LD Targeting of the M-domain in Cells.

Figure 4

(A) Analysis of the LD-targeting ability of a series of M-domain mutants. Drosophila cells were transfected with mCherry-tagged constructs and incubated 14-18 hours with 0.5 mM oleic acid. LDs were stained with BODIPY. Representative images are shown. Scale bar, 5 μm (merge), 1 μm (inlay).

(B) Quantification of the protein signal on droplets. Data are represented as mean + SD. At least 10 cells were analyzed in each independent experiment.