Fig. 8.

HSP70 promotes nuclear accumulation of Ad5 hexon but has no effect on late promoter activity. (a) HelaΔII cells were treated with HSP70 or control siRNA as in Fig. 6(c), then infected with Ad5 wt300 at m.o.i. of 5 for 16 h. Cytoplasmic and nuclear fractions were analysed by SDS-PAGE and Western blotting. Replicate blots were probed with antibodies to the proteins indicated and bands quantified as in Fig 5, normalized to GAPDH (cytoplasmic) or SP1 (nuclear). (b) Hela cells were treated with siRNA as in (a) and then transfected with MLP or L4P luciferase reporter. Luciferase activity was measured after 20 h and normalized to a β-galactosidase transfection control as described by Wright et al. (2015). (c) HelaEV and HelaΔII cells were infected with wild-type Ad5 at m.o.i. of 5, and total protein extracts analysed by Western blotting at 20 h post-infection for the proteins indicated.