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. 2016 Nov 10;97(11):2883–2893. doi: 10.1099/jgv.0.000608

Fig. 2.

Fig. 2.

E1E2 genes used to generate E1E2-matched HCVcc and HCVpp are genetically diverse. Maximum-likelihood tree of E1E2 amino acid sequences of envelope-matched HCVcc and HCVpp (diamonds) with 634 genotype 1 reference sequences from GenBank (lines without symbols). Nineteen primary isolate E1E2 genes that were functional in HCVpp (12 genotype 1a and 7 genotype 1b) were used to generate HCVcc chimeras: 13 HCVcc chimeras were replication competent (red symbols) and 6 were replication incompetent (blue symbols). These novel replication-competent HCVcc as well as two previously described HCVcc chimeras, H77/JFH-1 (genotype 1a E1E2) and S52/JFH-1 (genotype 3a E1E2) (green symbols), were used in infectivity and neutralization experiments.