Fig. 6.

Positive correlation between the rank orders of F_u values measured using HCVpp and IC₅₀ values measured using E1E2-matched HCVcc. E1E2-matched HCVcc and HCVpp with six different E1E2 proteins (H77, 1a38, 1a53, 1b09, 1b52 and S52) were tested in duplicate in neutralization assays with up to seven different bNabs or control non-specific IgG (34 total bNAb/E1E2 combinations). For HCVpp, neutralization was tested at a single concentration of mAb (10 µg ml⁻¹), and F_u was calculated (infection in the presence of 10 µg ml⁻¹ of mAb/infection in the presence of non-specific IgG). HCVcc were tested with serial dilutions of these bNabs and IC₅₀ values were calculated from these curves. Values are summarized in Table 2. Relative HCVpp F_u values were compared to IC₅₀ values of E1E2-matched HCVcc neutralized by the same bNAbs. Each point indicates the HCVcc IC₅₀ on the x-axis and HCVpp F_u on the y-axis of E1E2-matched HCVcc and HCVpp. Colours indicate mAb tested (cyan, AR3C; pink, AR5A; light blue, AR4A; dark blue, CBH-5; purple, HC33.4; green, HC84.22; red, HC84.26; orange, AR3A) and shapes indicate E1E2 (○, 1a09; ▲, 1a38; □, 1a53; ⚫, H77; △, 1b52; ■, 1b09; ×, S52). Correlation (r) and P value were computed using the Spearman method.