Fig. 3.

Branched-chain amino acids (bcaa) and/or insulin depletion did not affect cell number (A) or cell allocation variables (B) of expanded blastocysts in mice (n = number of blastocyst analysed) 0.14 replicates (2–4 females per replicate) (ANOVA). 2-cell embryos were in vitro cultured up to the blastocyst stage in either 100% (N = Normal) or 50% (L = Low) serum insulin levels with either 100% or 50% of bcaa concentrations found in the uterine luminal fluid of well-fed mice.