Fig 3. Confirmation of differential expression of IFNs in tissues by RT-qPCR and at the protein level in plasma.
(A) IFN-α mRNA expression in PLNs by RT-qPCR. (B) IFN-β mRNA expression in PLNs by RTqPCR. (C) IFN-α mRNA expression in RB by RT-qPCR. (D) IFN-γmRNA expression in PLNs by RT-qPCR. (E) MXA mRNA expression in PLNs by RT-qPCR. (F) IRF9 mRNA expression in PLNs by RT-qPCR. (G) Type-I IFN antiviral activity measured in plasma (MDBK/VSV biological assay) and (H) plasma IFN-γ concentration (measured by Luminex). ULD = Under the Limit of Detection. The Lower Limit of Quantification (LLOQ) is represented as a red dotted line in figure 3H. P values are given for non-parametric Wilcoxon rank sum test and was considered significant if p < 0.05.