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(A) Tet KD by pdf-gal4 does not affect organization of PDF neurons in adult brain. Brains are stained by anti-PDF antibody. (B) Tet-GFP is not expressed in PDF neurons in adult males or, (C) in third instar larval brains. (D) Tet-GFP protein is observed in the cytoplasm of PDF positive neurons in the embryonic central nervous system (stage 16).
