Fig 6. Tet controls ovarian development.
(A) The ovarioles are clearly separated and contain up to stage 8 egg chambers (white arrows). Tet mutant ovaries fail to separate their ovarioles, but do contain some disorganized egg chambers (orange arrows). The Tetnull ovaries does not show the clear separation of the somatic (TJ, red) and germline tissues. 1B1 in green outlines the somatic cells and fusome. (B) Left side, 3rd instar larval gonad: Tetnull gonad display the defect in cell organization. TJ (purple) labels the somatic cells and Vasa (red) labels the germline cells. Right side, Tet-GFP is observed in terminal filament cells in WT late 3rd instar larval ovaries. Red, filamentous actin, most highly expressed around the terminal filament cells. The germline clones induced in 0-24h embryogenesis showed a strong reduction ((p < 0.0001) (C), but not the ones induced at 3rd instar larval stage ((p = 0.11) (D). PGC, primordial stem cells; GSC, germ line stem cells.