Table 1. Deduced functions of ORFs in the phthoxazolin A biosynthetic gene cluster.
| Gene | Sizea | Homologb and origin | Identity/ similarity (%) | Proposed function |
|---|---|---|---|---|
| orf 5 | 504 | GlpK (WP_015654981), Streptomyces davawensis JCM 4913 | 91/95 | Glycerol kinase |
| orf 6 | 255 | ASC56_RS09905 (WP_055490882), Streptomyces sp.TP-A0356 | 92/96 | IclR-family transcriptional regulator |
| orf 7 | 142 | ADL25_RS11400 (WP_059127556), Streptomyces sp. NRRL F-5122 | 67/76 | Histidine kinase |
| orf 8 | 80 | IQ62_RS20385 (WP_037697207), Streptomyces scabiei NCPPB 4086 | 85/91 | Hypothetical protein |
| orf 9 | 333 | Ppk2 (WP_007385561), Streptomyces sviceus ATCC 29083 | 88/93 | Polyphosphate kinase |
| orf 10 | 180 | SAMN05216482_0059 (SEB58800), Streptomyces sp. PAN_FS17 | 78/87 | Hypothetical protein |
| orf 11 | 481 | G412_RS0110405 (WP_02881204), Streptomyces flavidovirens DSM 40150 | 96/98 | Glyceraldehyde 3-phosphate dehydrogenase |
| orf 12 | 137 | NF37_RS0107960 (WP_032755078), Streptomyces alboviridis NRRL B-1579 | 81/91 | Integrase |
| orf 13 | 501 | AWV61_RS50755(WP_060880896), Streptomyces scabiei 95–18 | 83/85 | Transposase |
| orf 14 | 188 | AVL59_RS26005 (WP_067308799), Sreptomyces griseochromogenes ATCC 14511 | 81/86 | Two-component system sensor kinase |
| orf 15 | 113 | CCN44_RS40620 (WP_086704188), Streptomyces tricolor NRRL B-16925 | 86/86 | Two-component system response regulator |
| orf16 | 94 | IG08_RS0113085 (WP_030600335), Streptomyces fulvoviolaceus NRRL B-2870 | 87/88 | LuxR-family transcriptional regulator |
| orf 17 | 169 | BIV24_RS13170 (WP_071366454), Streptomyces sp. MUSC 93 | 74/82 | Polyketide cyclase |
| ptxA | 1065 | OzmM (ABS90474), Streptomyces albus JA3453 | 57/68 | Acetyl transferase |
| ptxB | 5939 | OzmH (ABS90470), S. albus JA3453 | 53/62 | Hybrid NRPS-PKS |
| ptxC | 877 | OzmQ (ABS90478), S. albus JA3453 | 67/76 | Type I PKS |
| ptxD | 362 | OzmP (ABS90477), S. albus JA3453 | 77/88 | Hypothetical protein |
| ptxE | 1154 | OzmO (ABS90476), S. albus JA3453 | 53/62 | NRPS |
| ptxF | 4885 | OzmN (ABS90475), S. albus JA3453 | 51/60 | Type I PKS |
| ptxG | 3542 | NRPS (OMI35273), Streptomyces sparsogenes DSM 40356 | 65/74 | NRPS |
| ptxH | 2860 | PKS 1–1 (ADI03434), Streptomyces bingchenggensis BCW-1 | 63/72 | Type I PKS |
| orf 26 | 155 | SibV (ACN39745), Streptosporangium sibiricum DSM 44039 | 66/77 | Dioxygenase |
| orf 27 | 306 | BZL62_RS04865 (WP_086716558), Streptomyces angustmyceticus NRRL B-2347 | 68/78 | Hypothetical protein |
| orf 28 | 347 | AOK13_RS10670 (WP_055559528), Streptomyces luridiscabiei NRRL B-24455 | 81/87 | IMP dehydrogenase |
| orf 29 | 517 | BZL62_RS04825 (WP_086716551), Streptomyces angustmyceticus NRRL B-2347 | 79/86 | Acetolactate synthase |
| orf 30 | 295 | SAMN05444920_109123 (SEG95653), Nonomuraea solani CGMCC 4.7037 | 55/70 | Hydroxyacid dehydrogenase |
| orf 31 | 410 | BR98_RS37570 (WP_083976095), Kitasatospora azatica KCTC 9699 | 70/82 | Cytochrome P450 |
| orf 32 | 71 | WT80_RS35315 (WP_081087741) Burkholderia stagnalis MSMB774WGS | 43/56 | unknown |
| orf 33 | 526 | KCH_RS21250 (WP_084223811), Kitasatospora cheerisanensis KCTC 2395 | 66/77 | Fatty acid Co A ligase |
| orf 34 | 409 | SAMN05216533_5065 (SEF00159), Streptomyces sp. Ag109_O5-10 | 85/92 | 5-Aminolevulinate synthase |
| orf 35 | 509 | Ann2 (AGY30678), Streptomyces calvus ATCC 13382 | 67/81 | 5-Aminolevulinate synthase |
| orf 36 | 225 | ColR1 (AIL50186), Streptomyces aureus SOK1/5-04 | 50/62 | LuxR-family transcriptional regulator |
| orf 37 | 248 | IF01_RS0119920 (WP_051755722), Streptomyces purpeofuscus NRRL B-1817 | 75/83 | TetR-family transcriptional regulator |
| orf 38 | 516 | OO66_RS31780 (WP_051763676), Streptomyces virginiae NRRL B-8091 | 74/83 | Multidrug MFS (major facilitator superfamily) transporter |
a Numbers refer to amino acid residues.
b Parenthetical codes are National Center for Biotechnology Information accession numbers.