Fig 5. Presence of GR-deficient cells in the inflamed colon during DSS-induced colitis in GR^flox and GR^lysM mice.

Mice received 2% DSS in the drinking water for 8 days and were sacrificed on day 12. (A) Representative photomicrographs from 2 μm colonic tissue sections incubated with an anti-GR antibody at 64x magnification. Comparable areas of severe inflammation are depicted for GR^flox and GR^lysM mice. The scale bar corresponds to 50 μm. (B) DNA was isolated from colonic tissue and the genomic region containing the loxP sites was amplified to detect recombination of the GR locus. PCR products were separated on an agarose gel. The analysis of two samples from each genotype is depicted. The “flox” band refers to the unrecombined GR locus, the “null” band refers to the recombined gene unable to encode an intact GR protein.