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. 2018 Jan 11;13(1):e0191040. doi: 10.1371/journal.pone.0191040

Fig 5. VEGF produced by decidualized endometrial stromal cells stimulate macrophage migration.

Fig 5

A-C, transwell migration assay showing migration of M0 cells without (A) or with (B) VEGF treatment (2 μg/mL). Bar graph (C) shows quantitative estimation of the number of migrated cells. Addition of VEGF to the medium in the lower chambers induced about a 3-fold increase in migration of cells (B and C). D-G, transwell migration assay of M0 cells; D, treatment with non-decidualized stromal cell-conditioned medium; E-F, treatment with decidualized stromal cell-conditioned medium in the absence (E) or presence (F) of the VEGF inhibitor sFlt1; G, quantification of migrated cells. Note that decidualized stromal cell-conditioned medium significantly stimulated migration of M0 cells (E and G) but the effect was completely abrogated by the addition of sFlt1 (F), suggesting that VEGF secreted into the conditioned medium may play a role in stimulating the migration of these cells. Graphs represent means ± standard errors; * = p<0.05, ** = p<0.01, *** = p<0.001. Scale bars represent 50 μm.