Fig 4. Investigation of DISC1-VHH complex formation by SAXS.
(A) Primary scattering data (Intensity I as a function of momentum transfer q, the latter being defined as 4π sinθ / λ) for the VHH domain (blue), the DISC1691-836 protein (red), and their complex (yellow). The inset shows the linear regions of the respective Guinier plots at low q values, as suggested by AUTORG; the slope of the linear fit is related to the radius of gyration of the respective sample. (B) Normalized Kratky plot of the data shown in (A). A peak at low qRg values is considered typical of folded globular proteins. (C) Distance distribution functions derived from the data shown in (A), using the indirect Fourier transformation implemented in DATGNOM. For each species, the intersection with the positive abscissa corresponds to the maximum particle diameter. A.u., arbitrary units.