Fig 5. Interaction of L3V5 viral polymerase with WT and mutant N proteins.

BSR-T7/5 CL21 cells were transfected with mutant or WT pTM1-N and pTM1-L3V5. Negative control was transfected pTM1-N without pTM1-L3V5 (Con). After 24 hours, cells were lysed and the lysate was applied to magnetic beads carrying V5 antibody. The bound L protein was dissociated from the beads and analysed by western blot. The proteins were fractionated on a 4–12% Bis-Tris plus (Novex) gel and transferred to a nitrocellulose membrane. Subsequently the blot was probed with anti-V5 antibody and RVFV anti-N antibodies and visualised using LI-COR. This image is representative of three repeats.