Fig 6. Activity of RVFV N protein mutants in a VLP assay.

BSR-T7/5 CL21 cells were transfected with pTM1-L, pTVT7-GM:hRen, pTM1-M, pTM1-FF-Luc and mutant or WT pTM1-N as described. Negative control cells were transfected with full complement of plasmids without pTM1-N (Con). After 48 hours, the supernatant was harvested and Benzonase treated. The treated supernatant was applied to BSR-T7/5 CL21 cells transfected 24 hours prior with pTM1-N and pTM1-L. Rluc activity was measured at 24 h post-infection. Experiments were carried out in triplicate and repeated three times.