Figure 3. In vivo 2-photon calcium imaging of identified striosomes and matrix.

(A) Mash1-CreER;Ai14 mice were injected with AAV5-hSyn-GCaMP6s and 4 weeks later were implanted with a cannula. (B) Image of a striosome acquired with the 2-photon microscope, illustrating tdTomato labeling in red and GCaMP in green (scale bar: 100 µm) in the striatum of a trained mouse. (C–E) Higher magnification images of the region indicated in B (scale bar: 10 µm), shown for individual green (C), red (D) and merged (E) channels. Arrowheads indicate double-labeled cells. (F–H) Representative examples of striosomes imaged in three other trained mice (scale bars: 100 µm).