Table 3. (A) List of Delta, S(2D), S(3D), Z-offset, and zS(3D) (Z-offset subtracted) for the mean separation between CENP-T(M) and Hec1(9G3) in samples with or without large Z-offset.
(B) List of Delta, S(2D), S(3D), Z-offset, and zS(3D) in samples with #1, #1.5, and #2 coverslips. The value zS(3D) was calculated from the S(3D) data after subtraction of the mean Z-offset in the middle of the spindle from the Sz values. All values were mean ±SD.
| A | Green | Red | Delta nm) | S(2D)(nm) | S(3D) (nm) | Z-offset (nm) | zS(3D) (nm) | ||
|---|---|---|---|---|---|---|---|---|---|
| Example 1 | CENP-T (M) | 9 G3 | 59.5 ± 6.3 | 60.8 ± 8.8 | 102.4 ± 26.7 | 79.7 ± 32.9 | 68.9 ± 10.2 | ||
| Example 2 | CENP-T (M) | 9 G3 | 57.3 ± 7.3 | 60.1 ± 11.7 | 64.8 ± 11.3 | 6.9 ± 23.2 | 64.5 ± 10.8 | ||
| B | Thickness | Green | Red | Delta nm) | S(2D)(nm) | S(3D) (nm) | Z-offset (nm) | zS(3D) (nm) | |
| Experiment 1 | #1 | CENP-T (M) | 9 G3 | 60.4 ± 6.9 | 63.5 ± 12.4 | 75.2 ± 17.6 | −28.6 ± 31.1 | 70.4 ± 14.2 | |
| Experiment 2 | #1 | CENP-T (M) | 9 G3 | 59.2 ± 10.0 | 62.6 ± 11.7 | 72.6 ± 16.3 | −18.3 ± 33.9 | 70.6 ± 14.6 | |
| Experiment 1 | #1.5 | CENP-T (M) | 9 G3 | 57.3 ± 7.3 | 60.1 ± 11.6 | 64.8. ± 11.3 | 6.9 ± 23.2 | 64.5 ± 10.8 | |
| Experiment 2 | #1.5 | CENP-T (M) | 9 G3 | 57.0 ± 7.1 | 60.6 ± 11.1 | 64.0 ± 11.6 | 6.0 ± 20.0 | 63.8 ± 11.2 | |
| Experiment 1 | #2 | CENP-T (M) | 9 G3 | 59.6 ± 7.7 | 62.1 ± 11.9 | 82.1 ± 27.2 | 46.4 ± 36.4 | 69.6 ± 21.9 | |
| Experiment 2 | #2 | CENP-T (M) | 9 G3 | 59.8 ± 7.4 | 62.5 ± 10.9 | 74.2 ± 16.2 | 28.7 ± 30.2 | 69.4 ± 10.8 |