Table 5. The list of measured values for Delta, ML2D, ML3D, dDelta, dML2D, and dML3D using protocols in Table 4.

The dDelta, dML2D, dML3D were differences from Method 1 (PFA fixation). All values were mean ±SD. n > 200 individual kinetochores for each experiment.

	Red-Green = mean ± S.D. nm
	Delta (nm)	ML2D (nm)	ML3D (nm)	dDelta (nm)	dML2D (nm)	dML3D (nm)
Method 1	57.2 ± 7.3	59.1 ± 11.6	62.6 ± 11.5	0	0	0.0
Method 2	56.8 ± 6.0	58.6 ± 8.8	61.3 ± 8.6	−0.4	−0.5	−1.3
Method 3	50.1 ± 7.1	52.8 ± 8.5	56.4 ± 8.2	−7.1	−6.3	−6.2
Method 4	58.7 ± 9.1	61.5 ± 12.4	63.1 ± 22.9	1.5	2.4	0.5
Method 5	55.2 ± 8.9	56.9 ± 11.7	61.3 ± 15.5	-2	−2.2	−1.3
Method 6	57.2 ± 9.0	60.0 ± 12.9	63.5 ± 21.6	0	0.9	0.9
Method 7	54.0 ± 10.0	57.2 ± 12.1	57.7 ± 12.2	−3.2	−1.9	−4.9
Method 8	46.4 ± 5.3	48.3 ± 7.7	52.0 ± 7.7	−10.8	−10.8	−10.6
Method 9	51.8 ± 7.4	53.8 ± 9.7	56.7 ± 10.0	−5.4	−5.3	−5.9