Fig. 1.

Enhanced mutation detection in cancer using tumor cells propagated under reprogramming conditions. A) Experimental scheme for DNA sequencing. DNA from blood was used as a source for germline DNA sequencing. Staining with EpCAM/Jam-A antibodies allowed separation of epithelial cells from stromal cells. See Table S1 for additional details of metastatic samples. B) Venn diagram showing mutations shared between unprocessed tumor and tumor-derived cells as well as mutations found exclusively in cultured cells. Data from two representative samples are shown. Details are in Table S2. C) Validation of FOXO1 mutation in the LCIS sample using droplet digital PCR. One-dimensional plots for both wild type (VIC) and mutant (FAM) -specific probes are shown from different tumor samples, water, and positive plasmid controls (separated by yellow vertical lines). The sample named C01 is the LCIS sample, which showed amplification with mutant-specific primers.