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. Author manuscript; available in PMC: 2019 Jun 1.
Published in final edited form as: Environ Sci Pollut Res Int. 2017 Jul 11;25(17):16481–16492. doi: 10.1007/s11356-017-9676-z

Fig. 2.

Fig. 2

PCB126 induces a proinflammatory response in human preadipocytes. Non-differentiated (NOD) preadipocytes (black bars) or differentiated (DIF) adipocytes (gray bars) were treated with 0, 0.5, or 10 μM PCB126 for 4 days and RNA was isolated (see diagram, figure 1a). Transcript levels of different cytokines were assessed by quantitative RT-PCR. All values represent the fold change (calculated as described in the Materials and Methods) compared to vehicle-treated NOD preadipocytes. Three assay replicates were performed and averaged. To more completely show the data and to demonstrate the differences between NOD and DIF, the left-hand y-axis indicates the calculated fold-change for NOD and the right-hand axis indicates the fold change for DIF. (A–E) inflammatory cytokines IL-8, MCP-1, IL-6, TNFα, and IL-1β. (F) Anti-inflammatory cytokine IL-10. Statistics comparing PCB-treated to vehicle-treated were performed as described in the Materials and Methods. **=p<0.01; ***=p<0.001; ns=non-significant.