Fig. 6.

The NF-κB inhibitor Bay11-7082 partially ameliorates the effects of PCB126 on adipogenesis. (A) Preadipocytes were treated for 4 days in vehicle only, PCB126 only, Bay11-7082 and PCB126, or Bay11-7082 only and then differentiated for 9 days (see diagram, figure 1c). Differentiated cultures were fixed and stained with AdipoRed, which has the highest fluorescence and lowest background in a green wavelength. Fluorescent photos were taken at 200X. (B) Fluorescence was quantified in four different fields using ImageJ software. Percentage is relative to vehicle-treated cells. (C) Preadipocytes were treated with indicated compounds and collected for RNA (see diagram, figure 1b) followed by assessment of IL-8 transcript. Fold-changes over vehicle-treated cells were calculated as described in the Materials and Methods. Three assay replicates were averaged. Statistics comparing treatments were performed as described in the Materials and Methods. **=p<0.01; ***=p<0.001.