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. 2018 Jan 11;8:544. doi: 10.1038/s41598-017-18944-0

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of BD,FO, FS and FSM given as diet twice daily for three weeks on MMP-2 growth factor expression in EAC-bearing mice. (A) Immunohistochemical staining of MMP-2 in EAC solid tumor sections (160×). BD (I), FO (II), FS (III) and FSM (IV). (B) Quantification of MMP-2 immunopositive cells to the total area of the microscopic field across six fields. Values were given as means ± SD (n = 6), a, b and c: Significantly different from BD, FO and FS respectively at p < 0.05 using ANOVA followed by Tukey-Kramer as post-hoc test.