Figure 1.

Treg subsets exhibit distinct yields and ex vivo suppressive capacities. (A) nvTregs, DR⁻mTregs, and DR⁺mTregs differ in yield when FACS-sorted from fresh donor blood (n = 7). (B) Typical suppression by CFSE dilution, is shown for co-cultures established with CFSE labeled Tresp and half as many Tregs (5 days). (C) Treg co-cultures were established under different stimulation protocols in which the cells were stimulated with αCD3/αCD2 (top), αCD3/αCD28 (middle) or anti-CD3/APC (bottom). The DR⁺mTregs induced the highest suppression, followed by DR⁻mTreg and lastly by the least suppressive nvTregs. Data shown are mean ± SEM, and significance determined by Student’s t test, is indicated as (*P < 0.05) and (**P < 0.01). [turn scatter plots into bar graphs with X+/− SEM].