Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Jan 11;8:542. doi: 10.1038/s41598-017-18863-0

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Induction of ADCC activity in vaccine recipients. Antibody-dependent cellular cytolysis (ADCC) activity was measured using CEM.NKr-CCR5 target cells coated with SF162 gp140 (5 µg/100,000 cells/sample), labeled with TL4 dye, and treated with diluted plasma (1:200) and with rhesus CD16⁺ human NK KHYG-1 effector cells at an effector/target ratio of 5:1. The mixtures were then suspended in a granzyme B (GzB) substrate for 30 min at 37 ^oC. GzB delivered into target cells by effector cells was detected with the fluorogenic GzB substrate and visualized by flow cytometry. ADCC was calculated based on percentage of GzB⁺ target cells above negative control. Cut-off value (dotted line) was determined as the mean plus 3 standard deviations of % GzB⁺ cells in pooled prebleed plasma. (a) ADCC activities were measured in plasma of 5 VAX003 and 2 VAX004 vaccinees collected at early (A05, after 2 vaccine doses), mid- (A07 or A09, after 3 or 4 doses), and final (A15, after 7 doses) time points. Averages and standard deviation of duplicate wells from an experiment are shown. (b) ADCC activity was detected at mid-time points (A07 or A09) but declined at A15. **P > 0.01; by the paired 2-tailed t test. (c) ADCC activity strongly correlated with anti-gp120 Ab levels detected in ELISA. Correlation analysis was performed using the nonparametric Spearman test.