Figure 1.

Transient cold-induced increase in cytosolic Ca²⁺ is lower as a result of the mca mutation. (A) Relative luminescence of plants harboring aequorin was measured before and after the addition (indicated by the vertical arrow) of precooled solution (3 °C or 10 °C) or room temperature solution (22 °C). The figures are of representative data. The peak luminescence after the addition of solution at 3 °C (B, n ≥ 10), 10 °C (C, n ≥ 9), and 22 °C (D, n ≥ 17) is shown. Data represent the means ± SD. n indicates the number of seedlings. *p < 0.05; **p < 0.005 versus the wild type. Significance was determined using unpaired Student’s t tests.