Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Jan 11;8:550. doi: 10.1038/s41598-017-17483-y

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

The expression levels of several cold-regulated genes, which are not regulated by CBF/DREB1, were reduced in the mca1 mca2 double mutant. (A) Relative mRNA transcript levels of CBF/DREB1 and its regulon genes, COR15A, COR47, and RD29A, in wild-type and mca1 mca2 plants were determined by quantitative RT-PCR analyses. Three-week-old plants grown at 24 °C were incubated at 4 °C for the indicated time. Values represent the means ± SD (n = 3) from representative experiments from 3 biologically independent experiments. (B) Relative expression levels of cold-regulated genes that are not CBF/DREB1-regulon genes³⁰ were determined by quantitative RT-PCR analyses. Values represent the means ± SD (n = 3) from representative experiments from 3 biologically independent experiments. (C) The expression levels of MCA1 and MCA2 in wild types and mca1 mca2 double mutants were investigated by quantitative RT-PCR analyses. An asterisk indicates a significant difference from wild-type plants at each point (p < 0.05) as determined by unpaired Student’s t-tests.