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. 2018 Jan 11;8:491. doi: 10.1038/s41598-017-18846-1

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Glucuronidase activity in cell lysates of recombinant S. lividans strains containing gusA under the control of the inducible P21-cmt promoter fused to different degradation tags. GUS wt – strain that contains wild type gusA gene; AAV - strain that contains GusA fused with AAV C-terminal tag; LVA – strain with GusA containing LVA C-terminal tag and ASV – strain with GusA fused to ASV C-terminal tag. The glucuronidase activity of the wild type GusA in the induced stage was denoted as 100%. The strains were grown in TSB medium for 2 days in the presence of the inducer, after which the biomass was collected, washed and grown in fresh TSB medium without the inducer for 2 days; at this point, the inducer was added again, and the strains continued to grow for 2 more days.