Table 2.
Primers used in this study.
| Primers | Sequence 5′-3′ | Purpose |
|---|---|---|
| XbagusAForw | AAAATCTAGATACGCATATGCTGCGGCCCGTCGAAACC | Cloning the gusA gene without RBS and promoter |
| EVgusARev | AAAAGATATCTGCTTCCCGCCCTGCTGCGG | |
| XbaRBSForw | AAAAGATATCGAAATCACTCCCAATTAATCTAG | Cloning randomly synthesized RBSs upstream of the gusA gene |
| NdeNRBSRev | AAAACATATGNNNNNNCCTCCNNNNNNNNNNTTTCTCATCCTAAAGAATCTCTC | |
| NdeWRBSRev | AAAACATATGWWWWWWCCTCCWWWWWWWWWWTTTCTCATCCTAAAGAATCTCTC | |
| NdeP72bezRBS | AAAACATATGTTTCTCATCCTAAAGAATCTCTC | Cloning the gusA gene with the P72 promoter but without RBS |
| RBSProg1Rev | AAAACATATGAATGAACCTCCTTCTTTCTTTTTCTCATCCTAAAGAATCTCTC | Cloning the gusA gene fused to in silico designed RBS |
| NdeRBS8Nrev | AAAACATATGTGTTTCCTCCAACGGTTCATTTTCTCATCCTAAAGAATCTCTC | Cloning gusA fused to synthetic RBSs |
| NdeRBS7Nrev | AAAACATATGTGTTCCTCCAACGGTTCATTTTCTCATCCTAAAGAATCTCTC | |
| NdeRBS6Nrev | AAAACATATGTGTCCTCCAACGGTTCATTTTCTCATCCTAAAGAATCTCTC | |
| NdeRBS5Nrev | AAAACATATGTGCCTCCAACGGTTCATTTTCTCATCCTAAAGAATCTCTC | |
| NdeRBS3NRev | AAAACATATGCCTCCAACGGTTCATTTTCTCATCCTAAAGAATCTCTC | |
| ApForw | TTTTTCTAGTTATATGAGTAAACTTGGTCT | Fusion the Apr gene with the P82 promoter |
| ApP82Rev | GCTACAATCCTACTTGAAGAATCCTAATTTTAGCCTCAGGAGACGAAAGGGCCTCGTGATA | |
| EGFPNRBSRev | TTTTGATATCGAACAGCTCCTCGCCCTTGCTGGATCGGGATCCTTTTTCGAACTGCGGGTGGCTCCACATNNNNNNCCTCCNNNNNNNNNGCTACAATCCTACTTGAAGAATC | Cloning randomly synthesized RBSs upstream of the egfp gene |
| EGFPNRBS-9For | TTTTTCTAGATCCTGAGGCTAAAATTAGGATTCTTCAAGTAGGATTGTAGCTGACTAATGGGAGGCGTCTGATGTGGAGCCACCCGCAGTTC | EGFP fusion with NRBS-9 |
| EGFPNRBS-131For | TTTTTCTAGATCCTGAGGCTAAAATTAGGATTCTTCAAGTAGGATTGTAGCATCGTAGGAGGAGGCAAAACATGTGGAGCCACCCGCAGTTC | EGFP fusion with NRBS-131 |
| EGFPRev | GATATCTTACTTGTACAGCTCGTCCATGC | |
| GusARevLVA | AAAAGATATCTTATCAGGCTACGAGGGCGAAGGCCTGCTGGGAGGAATCGCGC | gusA fusion with degradation tags |
| GusARevAAV | TTGGTGTTGGCCTGCTTCCCGCCCTGCTGCGGAAAAGATATCTTATCAAACGGCAGCGGCGAAGGCCTGCTGGGAGGAATCGCGCTTGGTGTTGGCCTGCTTCCCGCCCTGCTGCGG | |
| GusARevASV | AAAAGATATCTTATCATACGGAAGCGGCGAAGGCCTGCTGGGAGGAATCGCGCTTGGTGTTGGCCTGCTTCCCGCCCTGCTGCGG | |
| GusASpeForw | AAAAACTAGTCGAGCAACGGAGGTAC |