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. 2018 Jan 11;8:553. doi: 10.1038/s41598-017-18541-1

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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HSP70 knockdown efficiency, surface marker expression, and proliferation of hMSCs. (A) RT-PCR results from full DNA gel images in Figure S3 and (B) Western Blot (WB) results from a full WB membrane in Figure S4 and band density quantification of heated hMSCs transfected with a scrambled shRNA (control) and HSP70 shRNAs #1 to #6; (C) Representative dot plots of flow cytometric analysis of surface marker expressions of hMSCs transfected with scrambled shRNA (top row) and HSP70 shRNA#5 (bottom row). Fluorescence thresholds were determined by fluorescence intensities of hMSCs with negative control antibodies IgG-FITC and IgG-PE; (D) dsDNA contents of hMSCs during osteogenic differentiation at Day 6, 12, 19, and 26. N = 4. KD: knockdown; HS: heat shock.