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. 2018 Jan 11;8:553. doi: 10.1038/s41598-017-18541-1

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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HSP70 expression in hMSCs with HSP70 knockdown during osteogenesis and chondrogenesis. HSP70 expression measured by Real-time RT-PCR in hMSCs during growth and osteogenic differentiation with and without heat shock at Day 6 (A), 12 (B), 19 (C), and 26 (D). Representative images of immunohistochemical staining of HSP70 in pellet culture samples at day 17 and 19 during chondrogenesis (E); Scale bar: 50 $μ$ m (Chon: chondrogenic differentiation; HS: heat shock; KD: knockdown). *P < 0.05; **P < 0.01. N = 4.