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. 2018 Jan 11;8:553. doi: 10.1038/s41598-017-18541-1

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Comparison between samples of HSP70 knockdown and controls in terms of ALP activity (A), calcium deposition (B) and real time PCR data of gene expression of Runx2 (C) and Osterix (D) during osteogenic differentiation in hMSCs. One enzyme unit of ALP is the amount of enzyme which releases 1 nmol p-nitrophenol per 15 minutes. Each sample had 10⁴ seeded hMSCs. N = 4. S: scrambled; KD: HSP70 knockdown; HS: heat shock. *P < 0.05; **P < 0.01.