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. 2018 Jan 11;8:469. doi: 10.1038/s41598-017-18801-0

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Axial skeletal defects in Ach mice. Lateral X-rays of 3-week-old WT (A) and Ach mice with mild (B) to severe (C) kyphosis. Kyphosis index (D) and body lengths (E) are shown for WT and Ach mice at 3 weeks and 6 weeks of age. Average L5 pedicle lengths (F) and L4-L6 lengths (G) are shown for the 3-week and 6-week-old mice. The graphical data are represented as mean ± SEM (*0.01 < p < 0.05, ***0.0001 < p < 0.001, ****p < 0.0001).